周峰

周峰博士

上海市徐汇区东安路130号

西13号楼315室

邮编：200030

电子邮件: Zhou_feng@fudan.edu.cn

研究方向与特长

蛋白质组学以及相关的各项技术

国际领先水平的新一代的蛋白质组检测平台，在大大提高覆盖率的基础上，灵敏度比普遍使用的平台高两个数量级，极大地促进了蛋白质组学平台在临床医学研究中的应用。
利用高灵敏度的蛋白质组检测平台在临床样品当中直接检测与分析疾病中重要的蛋白质的调控网络。
利用临床样品，研究重大疾病当中蛋白质翻译后修饰如磷酸化，甲基化以及泛素化的动态变化，找出在重大疾病中起决定性作用的信号传导通路。

精通蛋白质组学为基础的高通量靶点药物筛选平台

利用新一代蛋白质组学平台研究传染性疾病的感染发展过程，并利用统计学方法分析蛋白质动态过程，高通量筛选药物靶点。
利用新一代质谱技术，检测蛋白质与小分子之间的相互作用，高通量筛选抑制蛋白质功能的药物。

研究经历

复旦大学附属上海医学院,复旦大学附属中山医院, 生物医学研究院,上海,中国

研究员(Principle Investigator)东方学者特聘教授, 2014年8月 - 至今

利用新一代的蛋白质组学平台研究造血干细胞到血红细胞的发育过程整个蛋白质组的动态过程，从而发现了大量不受转录调控的对发育过程起到重要作用的蛋白质，第一次揭示了转录后调控在血红细胞发育中的重要作用。
通过对 HIV 侵入 T 细胞的深度蛋白质组分析，找出受病毒特异影响的宿主细胞蛋白质，利用生物统计的方法找出重要的抑制 HIV 感染的蛋白质靶点。

哈佛医学院, 生物化学及分子药理学系,波士顿,马萨诸塞州

博士后（Research Fellow）,2007年8月– 2014年8月, 导师:Jarrod A. Marto, Ph.D.

研发了新一代蛋白质组检测平台，达到国际领先水平。在对蛋白质组覆盖率大大提高的同时，灵敏度提高了两个数量级，为蛋白质组学在临床医学研究中的应用打下了坚实的基础。
研发了超高灵敏度蛋白质相互作用检测平台。

University of Delaware, Department of Chemistry and Biochemistry, Newark, DE

博士研究生(Research assistant), 2002年7月 – 2007年7月, 导师: Murray V. Johnston, Ph.D.

开发并利用CIEF-RPLC-MS技术，对未酶切过的整个蛋白质进行分析
利用自己开发的CIEF-RPLC-MS平台分析了绿硫菌的蛋白质组
开发了基于采样锥的Top-down蛋白质测序技术。

复旦大学, 化学系,上海,中国

研究助理(Research assistant), 1999年8月 – 2002年6月，导师: 杨芃原

发明了双喷头纳升电喷雾系统，并利用该系统对蛋白质的酶切肽段进行了高精度分析。
设计并制作了基于自适应人工神经网络的智能嗅觉系统，并用该系统检测了各个环境中的甲醛，氟利昂等有毒有害气体。

学历

University of Delaware, Newark, DE

Ph.D., Analytical Chemistry, August 2002 – June 2007

复旦大学, 上海, 中国

学士, 分析化学, 2000年6月

著作

Zhou F, Johnston MV. Hypersensitive measurement of proteins by capillary isoelectric focusing and LC/MS, in: Chromatographic Methods in Clinical Chemistry and Toxicology, editors R. Bertholf and R. Winecker, John Wiley and Sons, New York.

研究论文

Zhou F, Lu Y, Ficarro SB, Adelmant GO, Luckey CJ, Marto JA. Genome-scale proteome quantification by DEEP SEQ mass spectrometry. Nature Communications. DOI: 10.1038/ncomms3171. IF: 10.005
Zhou F, Lu Y, Ficarro SB, Webber JT, Marto JA. A nanoflow low pressure high peak capacity single dimension LC-MS/MS platform for high throughput in-depth analysis of mammalian proteomes. Analytical Chemistry. 2012: 84(11): 5133-5139. Times Cited: 4. IF: 5.695
Caro P, Kishan AU, Norberg, E, Stanley IA, Chapuy B, Ficarro SB, Polak K, Tondera D, Gounarides J, Yin H, Green MR, Zhou F, Chen LF, Monti S, Marto JA, Shipp MA, Danial NN. Metabolic Signatures Uncover Distinct Targets in Molecular Subsets of Diffuse Large B Cell Lymphoma. Cancer Cell 2012:22, 547-560. Times Cited: 10. IF: 24.755
Zhou F, Sikorski TW, Ficarro SB, Webber JT, Marto JA. Online nanoflow reversed phase-strong anion exchange-reversed phase liquid chromatography-tandem mass spectrometry platform for efficient and in-depth proteome sequence Analysis of Complex Organisms. Analytical Chemistry. 2011;83(18):6996-7005. Times Cited: 15. IF: 5.695
Zhou F, Cardoza JD, Ficarro SB, Adelmant GO, Lazaro JB, Marto JA. Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage. J Proteome Res. 2010;9(12):6242-55.Times Cited: 21. IF: 5.056
Zhou F, Hanson TE, Johnston MV. Intact protein profiling of C. tepidum by capillary isoelectric focusing, reversed phase liquid chromatography and mass spectrometry with pI determination. Analytical Chemistry 2007, 79: 7145-7156. Times Cited: 32. IF: 5.695
Zhou F, Johnston MV. Protein profiling by capillary isoelectric focusing, reversed phase liquid chromatography and mass spectrometry. Electrophoresis 2005, 26: 1383-1388. Times Cited: 33. IF: 3.261
Ginter JM, Zhou F, Johnston MV. Generating protein sequence tags by combining cone and conventional collision induced dissociation in a quadrupole time-of-flight mass spectrometer. Journal of the American Society for Mass Spectrometry 2004, 15: 1478-1486. Times Cited: 26. IF: 3.592
Zhou F, Johnston MV. Protein characterization by on-line capillary isoelectric focusing, reverse phase liquid chromatography and mass spectrometry. Analytical Chemistry 2004, 76: 2734 – 2740. Times Cited: 64. IF: 5.695
Zhou F, Shui WQ, Lu Y, Yang PY, Guo YL. High accuracy mass measurement of peptides with internal calibration using a dual electrospray ionization sprayer system for protein identification. Rapid Communications in Mass Spectrometry 2002, 16: 505-511. Times Cited: 22. IF: 2.509
Lu Y, Zhou F, Shui WQ, Bian LP, Yang PY. Pulsed electrospray for mass spectrometry. Analytical Chemistry 2001, 73: 4748-4753. Times Cited: 17. IF: 5.695
Wei JF, Shui WQ, Zhou F, Lu Y, Chen KK, Xu GB, Yang PY. Naturally and externally pulsed electrospray. Mass Spectrometry Reviews 2002, 21: 148-162. Times Cited: 37. IF: 7.735
Zhang S, Huang F, Zhou F, Zhao JW, Wen LJ, Yang PY. Determination of thiols in urinary sample by capillary-column liquid chromatography with amperometric detection at a carbon electrode. Talanta 2002, 58: 451-458. Times Cited: 10. IF: 3.498
Gao F, Zhao JW, Zhang S, Zhou F, Jin W, Zhang XM, Yang PY, Zhao DY. Application of C-18-modified mseoporous SBA-15 as the substrate for high performance liquid chromatography. Chemical Journal of Chinese Universities-Chinese 2002, 23: 1494-1497. Times Cited: 9. IF: 0.856
Wen LJ, Zhang LY, Zhou F, Lu Y, Yang PY. Quantitative determination of Freon gas using an electronic nose. Analyst 2002, 127: 786-791. Times Cited: 1. IF: 3.969
Zhang LY, Wen LJ, Zhou F, Zhang S, Yang PY. Electronic nose for the determination of formaldehyde in air. Chemical Journal of Chinese Universities-Chinese 2003, 24: 1381-1384. Times Cited: 3. IF: 0.856
Lu Y, Bian LP, Zhou F, Sun P, Yang PY, Guo YL, Yu CT. Preliminary research on pulsed-electrospray phenomenon. Chemical Journal of Chinese Universities-Chinese 2001, 22: 560-562. Times Cited: 3. IF: 0.856
Zhou J, Li P, Zhang S, Long YC, Zhou F, Huang YP, Yang PY, Bao MH. Zeolite-modified microcantilever gas sensor for indoor air quality control. Sensors and Actuators B-Chemical 2003, 94: 337-342. Times Cited: 70. IF: 3.535

大会报告

Zhou F., Johnston, M.V., "Protein Profiling by capillary isoelectric focusing, reversed phase liquid chromatography and mass spectrometry." Oral presented at the 26th Annual East Coast Ion Chemistry Conference, Newark, DE. October 2004.
Zhou F., Johnston, M.V., "Intact protein profiling of C. tepidum by capillary isoelectric focusing, reversed phase liquid chromatography and mass spectrometry." Poster presented at the 54th Annual American Society for Mass Spectrometry Conference, Seattle, WA. May 2006.
Zhou F., Johnston, M.V., "Protein Profiling by capillary isoelectric focusing, reversed phase liquid chromatography and mass spectrometry." Poster presented at the 53rd Annual American Society for Mass Spectrometry Conference, San Antonio, TX. June 2005.
Zhou F., Johnston, M.V., "Protein characterization by on-line capillary isoelectric focusing, reverse phase liquid chromatography and mass spectrometry." Poster presented at the 52nd Annual American Society for Mass Spectrometry Conference, Nashville, TN. May 2004.

专利

Zhou J, Li P, Huang YP, Zhang S, Zhou F, Yang PY, Zhao DY, inventors; Zeolite-modified microcantilever biological sensor. Chinese patent 2504633. 2002 Aug 7.
Wen LJ, Zhang LY, Zhou F, Bian LP, Lu Y, Zhang S, Yang PY, Zhang J, Zhang SX, Shen FD, inventors; Portable quantitative electronic nose. Chinese patent 1381721. 2002 Nov 27.
Zhang LY, Wen LJ, Zhou F, Zhang S, inventors; Structure of portable artificial nose. Chinese patent 2540633. 2003 Mar